| Congresso Brasileiro de Microbiologia 2023 | Resumo: 898-1 | ||||
Resumo:Urinary tract infections (UTIs) are the most common cause of bacterial infections in humans. It is estimated that about 150 million people worldwide are annually affected by UTI. The Gram-negative enterobacterium Escherichia coli (UPEC - Uropathogenic E. coli) is the most common causative agent of community-acquired UTIs being responsible for 80-90% of the cases. The ability of UPEC to survive in the urinary tract depends on its physiology, metabolism, and virulence determinants. Recently, it has been demonstrated that the transportation and metabolism of various carbohydrates are associated with virulence in enterobacteria. However, the precise role of carbohydrate utilization in the expression and regulation of bacterial virulence has not yet been determined. Therefore, we sought to understand the impact of different carbon sources on the fitness and virulence factors of four multidrug-resistant (MDR) UPEC (32, 76, 174, 214). To achieve this purpose, the MDR UPEC strains were subjected to antibiotic resistance profiling determination, and under incubation under different unique carbon sources we evaluated: (i) bacterial fitness (ii) type 1 fimbriae expression, (iii) biofilm production, and (iv) blood and serum bacterial survival. Under anaerobic conditions, UPEC strains utilized D-(-)-sorbitol as the carbon source presenting the lowest generation time. Three of the four strains (UPEC 76, 174, 214) did not use D-(-)-fructose as a carbon source for growth. Yeast Agglutination Assay suggests that D-(-)-fructose utilization is related to the expression of functional type 1 fimbriae by three strains (UPEC 32, 174, 214). UPEC 76 did not utilize D-(-)-fructose as a carbon source for growth or for expressing functional type 1 fimbriae. All UPEC strains were classified as moderate biofilm producers when cultivated under N-acetyl-D-glucosamine as a unique carbon source. Three strains (UPEC 32, 174, 214) exhibited an increased ability to survive in blood and serum when utilizing N-acetyl-D-glucosamine as the sole carbon source. Taken together, our data suggest that the metabolism of different carbon sources (sugars) modulates the expression of virulence factors such as excessive growth, expression of functional type 1 fimbriae, and serum resistance in different MDR UPEC strains. Palavras-chave: Uropathogenic Escherichia coli, Carbohydrate metabolisms, Virulence, Fitness, Type 1 fimbriae Agência de fomento:Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF) e Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||